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AWWA JAW60540 Journal AWWA - Sequential Disinfection of Adenovirus Type 2 with UV-Chlorine-Chloramine

Journal Article by American Water Works Association, 10/01/2004

Ballester, Nicola A.; Malley, James P., Jr.

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Bench-scale experiments determined the inactivation rates of adenovirus serotype 2 withlow-pressure, high-output ultraviolet (UV) light, chlorine (Clsub2/sub), and preformed chloramines.Studies with sequential chloramines were also done to mimic water treatment practices.Sequential experiments with adenovirus serotype 2 suspended in laboratory-grade waterand natural waters containing ammonia were exposed to either UV light followed byClsub2/sub/chloramines or the reverse sequence. Adenovirus log reductions were quantified throughcell culture techniques. A free Cl2 C T (concentration time) of 1.22 mg-min/L resulted in a3.72-log reduction, a preformed chloramine C T of 264.5 mg-min/L resulted in a 1.2-logreduction, a sequential chloramine C T of 40.5 mg-min/L resulted in a 1-log reduction, anda UV dose of 40 mJ/cmsub2/sub resulted in a 1-log reduction. Up to 4-log reductions were achievedwith a UV dose of 40 mJ/cmsub2/sub followed by a sequential chloramine C T of 27.2 mg-min/L.This suggests that sequential disinfection may be the best option for dealing with UV-resistantorganisms such as adenoviruses. Includes 39 references, figures.

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